現(xiàn)貨供應(yīng),歡迎訂購: : 細(xì)胞活性測定試劑盒產(chǎn)品信息: 產(chǎn)品名稱 | 貨號(hào) | 規(guī)格 | 儲(chǔ)存條件 | Cell Proliferation Kit(XTT based) 細(xì)胞活性測定試劑盒(二甲氧唑黃) | 20-300-1000 | 1000 assays | -20ºC |
廣泛應(yīng)用于細(xì)胞生物學(xué)的各類實(shí)驗(yàn)中,例如研究生長因子、細(xì)胞活素類和培養(yǎng)基組分,細(xì)胞毒性因子和淋巴細(xì)胞的活性。 MTT和XTT都是檢測細(xì)胞存活和生長的方法,MTT的產(chǎn)物為水不溶性的藍(lán)紫色結(jié)晶甲瓚(Formazan),并沉積在細(xì)胞中,需要加有機(jī)溶劑溶解,由于在去上清的操作時(shí)會(huì)有可能帶走小部分的甲瓚,故重復(fù)性略差。XTT作為線粒體脫氫酶的作用底物,被活細(xì)胞還原成水溶性的橙黃色甲臢產(chǎn)物,當(dāng)XTT與電子偶合劑(例如PMS)聯(lián)合應(yīng)用時(shí),其所產(chǎn)生的水溶性的甲臢產(chǎn)物的吸光度與活細(xì)胞的數(shù)量成正比。
XTT優(yōu)點(diǎn): 1. 使用方便,省去了洗滌細(xì)胞 2. 檢測快速 3. 靈敏度高,甚至可以測定較低細(xì)胞密度 4. 重復(fù)性優(yōu)于MTT 適用于單核細(xì)胞(Monocytes),人肝癌細(xì)胞Hep G2胚胎纖細(xì)胞 Ehd-1, 小鼠纖維細(xì)胞,惡性黑色素瘤A375,胰腺癌BXPC-3等30余種細(xì)胞類型。 更多相關(guān)產(chǎn)品: BIOLOGICAL INDUSTRIES 05-010-1A "DCCM-1 without L-Glutamine 哺乳動(dòng)物細(xì)胞懸浮培養(yǎng)無血清培養(yǎng)基" 500ml 4℃ BI公司 05-015-1A "DCCM-2 without L-Glutamine 哺乳動(dòng)物細(xì)胞懸浮培養(yǎng)無血清培養(yǎng)基" 500ml 4℃ BI公司 05-040-1A "Low Protein Media BSA-Free (LPM), without L-Glutamine 哺乳動(dòng)物細(xì)胞懸浮培養(yǎng)無血清培養(yǎng)基" 500ml 4℃ BioInd公司 01-015-5A Basal Medium Eagle (Earle's) 10X 500ml AMB BioInd公司 01-340-1B MEM Non-Essential Amino Acids Solution, 100X Conc. 100ml 4℃ BioInd公司 02-010-1A Earle's Balanced Salt Solution 500ml AMB BioInd公司 02-010-5A Earle's Balanced Salt Solution, 10X Conc., without Sodium Bicarbonate 500ml AMB BIOLOGICAL INDUSTRIES 02-010-5A Earle's Balanced Salt Solution, 10X Conc., without Sodium Bicarbonate 500ml AMB BIOLOGICAL INDUSTRIES 02-011-5B Earle's Balanced Salt 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