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Anti-Cytokeratin 18 C-04 antibody （ab668）抗細(xì)胞角蛋白18抗體
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產(chǎn)品型號(hào)：
 ab668
產(chǎn)品報(bào)價(jià)：
產(chǎn)品特點(diǎn)：
 Anti-Cytokeratin 18 [C-04] antibody (ab668)抗細(xì)胞角蛋白18抗體
貨號(hào)：ab668
廠家：Abcam公司
規(guī)格：100ug
保存溫度：-20℃ 和 2～8℃
貨期：現(xiàn)貨2～5天，期貨2-3周
  ab668Anti-Cytokeratin 18 C-04 antibody （ab668）抗細(xì)胞角蛋白18抗體的詳細(xì)資料：

咨詢：  （重慶） （貴陽）

Anti-Cytokeratin 18 [C-04] antibody (ab668)抗細(xì)胞角蛋白18抗體產(chǎn)品信息：

  • Product nameAnti-Cytokeratin 18 [C-04] antibody
  • Description

    Mouse monoclonal [C-04] to Cytokeratin 18

  • SpecificityHuman Cytokeratin
  • Tested applicationsICC, IHC-P, WB, IHC-FoFr, Flow Cyt, IHC-Fr, IP, ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Sheep, Goat, Horse, Hamster, Cow, Cat, Dog, Human, Pig, Marmoset (common)

  • Immunogen

    Tissue / cell preparation (Human). (Cytoskeleton preparation of epidermal carcinoma cell line A431).

  • Positive control
    • A431 cells

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4

  • Concentration 100 µg at 1 mg/ml

  • Purity>95% by SDS-PAGE
  • Purification notesPurified from culture supernatant using protein A-affinity chromatography.
  • Clonality Monoclonal
  • Clone numberC-04
  • IsotypeIgG1
  • Research Areas
    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins

Applications

Our Abpromise guarantee covers the use of ab668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

ApplicationNotes
ICCICC: Use at an assay dependent dilution.
IHC-PIHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WBWB: Use at an assay dependent dilution. Predicted molecular weight: 45 kDa.
IHC-FoFrIHC-FoFr: Use at an assay dependent concentration.
Flow CytFlow Cyt: Use 1µg for 106 cells. (also see PMID 18946470)
IHC-FrIHC-Fr: Use at an assay dependent dilution.
IPIP: Use at an assay dependent dilution.
ICC/IFICC/IF: Use at an assay dependent dilution.

Target

  • FunctionInvolved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity). When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
  • Tissue specificityExpressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
  • Involvement in diseaseDefects in KRT18 are a cause of cirrhosis (CIRRH) [MIM:215600].
  • Sequence similaritiesBelongs to the intermediate filament family.
  • Post-translational
    modificationsPhosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
    Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
    O-glycosylated at multiple sites; glycans consist of single N-acetylglucosamine residues.
  • Cellular localizationCytoplasm > perinuclear region.

  • Database links

    • Entrez Gene: 506480 Cow
    • Entrez Gene: 477601 Dog
    • Entrez Gene: 3875 Human
    • Entrez Gene: 16668 Mouse
    • Entrez Gene: 294853 Rat
    • Omim: 148070 Human
    • SwissProt: P05783 Human
    • SwissProt: P05784 Mouse

    see all

  • Alternative names

    • Cell proliferation inducing gene 46 protein antibody
    • Cell proliferation inducing protein 46 antibody
    • Cell proliferation-inducing gene 46 protein antibody

    see all

Anti-Cytokeratin 18 [C-04] antibody images

  • Flow Cytometry - Anti-Cytokeratin 18 antibody [C-04] (ab668)

    Flow Cytometry - Anti-Cytokeratin 18 antibody [C-04] (ab668)

    Overlay histogram showing HCT116 cells stained with ab668 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab668, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HCT116 cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Triton used under the same conditions.
     

  • Immunocytochemistry/ Immunofluorescence - Cytokeratin 18 antibody [C-04] (ab668)

    Immunocytochemistry/ Immunofluorescence - Cytokeratin 18 antibody [C-04] (ab668)This image is courtesy of an anonymous Abreview

    ab668 at 1/100 staining A431 Human epithelial carcinoma cells by ICC/IF. The cells were paraformaldehyde fixed, permeabilized with 0.25% Triton X-100 and then blocked with BSA prior to incubation with the antibody for 24 hours at 4°C. A FITC conjugated goat anti-mouse IgG antibody was used as the secondary. The right hand image shows staining with ab668 followed by secondary antibody. The left hand image shows DAPI staining with secondary only.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytokeratin 18 antibody [C-04] (ab668)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cytokeratin 18 antibody [C-04] (ab668)

    ab668 (2µg/ml) staining cytokeratin 18 in human skin using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of sweat coils.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 [C-04] antibody (ab668)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 18 [C-04] antibody (ab668)This image is courtesy of an Abreview submitted by Carl Hobbs

    ab668 staining Cytokeratin 18 in Cat lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/500 in TBS/BSA/azide) for 2 hours at 21°C. A Biotin-conjugated goat anti-mouse IgG polyclonal (1/200) was used as the secondary antibody.

    Coloured arrowheads indicate positivity.
    Good immunolabelling of bronchial tree epithelia (green), alveolar lining epithelium (red ).
    Goblet cells are negative (asterisk).
    Heavily stained structures are submucosal mucous glands.

  • Immunocytochemistry/ Immunofluorescence - Cytokeratin 18 antibody [C-04] (ab668)

    Immunocytochemistry/ Immunofluorescence - Cytokeratin 18 antibody [C-04] (ab668)Image courtesy of an anonymous Abreview.

    ab668 staining Cytokeratin 18 in horse primary bronchial epithelial cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in acetone and then blocked using 3% BSA for 10 hours at 4°C. Samples were then incubated with primary antibody at 1/100 for 1 hour at 22°C. The secondary antibody used was a goat anti-mouse conjugated to FITC used at a 1/200 dilution.

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    Abcamab11504Rat monoclonal [KT12] to TCR V beta 12  2ml3996TCR V beta 12hystemcell一抗

    Abcamab11505Mouse monoclonal [G3] to CD3 zeta （FITC）500ul3996TCR zeta chainhystemcell一抗

    Abcamab11506Mouse monoclonal [15F11] to gamma Catenin100ul3996gamma Cateninhystemcell一抗

    更多詳情，請(qǐng)：     

    重慶市華雅干細(xì)胞技術(shù)有限公司
產(chǎn)品相關(guān)關(guān)鍵字： ab668 細(xì)胞增殖誘導(dǎo)基因46蛋白抗體 細(xì)胞增殖誘導(dǎo)蛋白抗體 抗細(xì)胞角蛋白18抗體 細(xì)胞角蛋白
　如果你對(duì)ab668Anti-Cytokeratin 18 C-04 antibody （ab668）抗細(xì)胞角蛋白18抗體感興趣，想了解更詳細(xì)的產(chǎn)品信息，填寫下表直接與廠家聯(lián)系：

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